plenti hygro h2b mruby backbone Search Results


plenti hygro h2b mruby backbone  (Addgene inc)
93
Addgene inc plenti hygro h2b mruby backbone
Plenti Hygro H2b Mruby Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ampr plenti pgk hygror pgkh2b mirfp670 lentiviral  (Addgene inc)
92
Addgene inc ampr plenti pgk hygror pgkh2b mirfp670 lentiviral
Ampr Plenti Pgk Hygror Pgkh2b Mirfp670 Lentiviral, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmdg.2  (Addgene inc)
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pax2g  (Addgene inc)
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Addgene inc pax2g
Pax2g, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmvvsv-g  (Addgene inc)
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Addgene inc pcmvvsv-g
Pcmvvsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plenti cmv h2b green fluorescent protein gfp hygro  (Addgene inc)
95
Addgene inc plenti cmv h2b green fluorescent protein gfp hygro
(A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt <t>H2B</t> <t>GFP-labeled</t> CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).
Plenti Cmv H2b Green Fluorescent Protein Gfp Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nod.h2-b (nod.b10sn- h 2b /j)  (Charles River Laboratories)
90
Charles River Laboratories nod.h2-b (nod.b10sn- h 2b /j)
(A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt <t>H2B</t> <t>GFP-labeled</t> CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).
Nod.H2 B (Nod.B10sn H 2b /J), supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c3.sw-h2 b /snj mice  (Jackson Laboratory)
90
Jackson Laboratory c3.sw-h2 b /snj mice
(A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt <t>H2B</t> <t>GFP-labeled</t> CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).
C3.Sw H2 B /Snj Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c3h/heouj mice  (Jackson Laboratory)
90
Jackson Laboratory c3h/heouj mice
(A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt <t>H2B</t> <t>GFP-labeled</t> CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).
C3h/Heouj Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c.b10-h2 b /lilmcdj (balb.b; h-2 b)  (Jackson Laboratory)
90
Jackson Laboratory c.b10-h2 b /lilmcdj (balb.b; h-2 b)
(A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt <t>H2B</t> <t>GFP-labeled</t> CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).
C.B10 H2 B /Lilmcdj (Balb.B; H 2 B), supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b6.akr-h-2k mice  (Jackson Laboratory)
90
Jackson Laboratory b6.akr-h-2k mice
(A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt <t>H2B</t> <t>GFP-labeled</t> CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).
B6.Akr H 2k Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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female c3h.sw-h2 b /snj mice  (Jackson Laboratory)
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Jackson Laboratory female c3h.sw-h2 b /snj mice
(A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt <t>H2B</t> <t>GFP-labeled</t> CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).
Female C3h.Sw H2 B /Snj Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt H2B GFP-labeled CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).

Journal: bioRxiv

Article Title: Inter-Spheroid Proximity and Matrix Remodeling Determine CAF-Mediated Cancer Cell Invasion

doi: 10.1101/2025.04.24.648914

Figure Lengend Snippet: (A) Ibidi 8-well glass-bottom slide with a 3D collagen matrix containing multicellular tumor spheroids. A zoomed-in schematic represents multicellular luminal M7 breast cancer and CAF spheroids embedded in a 3D collagen matrix, separated by a distance “d.“ (B) Representative fluorescence images of M7 mCherry-labeled cancer cell spheroids (red) and 19tt H2B GFP-labeled CAF spheroids (white) taken at 0 h, 24 h, and 48 h post-embedding in a 3 mg/ml rat-tail collagen matrix (green). Spheroids were separated by distances of (ii) 450 µm and (iii) 150 µm. (C) Quantification of the M7 (MCF7) spheroid area was performed under three conditions: when embedded alone (n = 9), with 19TT spheroids within 200 µm (n = 9), and with 19TT spheroids at distances greater than 200 µm (n = 9) after 48 h. M7 spheroids seeded alone only doubled in size. Tumor spheroids seeded more than 200 µm away exhibited a 2.5-fold increase. In comparison, tumor spheroids seeded less than 200 µm from 19TT CAF spheroids demonstrated a nearly four-fold increase in area over 48 h (*p < 0.05, ****p < 0.0001). (D) Overview of (1) single-cell dissemination (yellow circles) observed from M7 spheroids and (2) collagen matrix integrity (degraded collagen area in yellow circles) under three conditions: (i) M7 alone, (ii) M7 + CAF, d ≥ D C , and (iii) M7 + CAF, d < D C , after 48 hours. (E) Void Fraction ( V f(t) / V f (0) ) Quantification of the collagen hydrogel measured from 0 to 48 h and normalzied to its initial value V f (0) for M7 (red) (n = 9), M7 + CAF, d ≥ D C (n = 9), (dark blue) and (iii) M7 + CAF, d < D C (light blue), after 48 h (n = 9).

Article Snippet: MCF7 cells were labeled with plv-mCherry, and 19TT cells were labeled with pLenti CMV H2B green fluorescent protein (GFP) Hygro (656-4). pLenti CMV GFP Hygro (656-4) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17446; http://n2t.net/addgene:17446 ; RRID:Addgene_17446).

Techniques: Fluorescence, Labeling, Comparison